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Fig. 2 | Molecular Medicine

Fig. 2

From: Hypoxia May Increase Rat Insulin mRNA Levels by Promoting Binding of the Polypyrimidine Tract-binding Protein (PTB) to the Pyrimidine-rich Insulin mRNA 3′-Untranslated Region

Fig. 2

Hypoxia increases insulin mRNA levels in isolated rat islets. Isolated rat islets were incubated for 6 hr (white bars) or 16 hr (black bars) in the presence of normoxia (21% O2) or hypoxia (10% or 5% O2) at 2.8 or 28 mM glucose, without (A) or with (B) 5 µg/ml actinomycin D. Insulin mRNA was detected by Northern hybridization and expressed per 28S rRNA and shown as percentage of control. Results are means ±SEM for six observations. *p < 0.05; ***p < 0.005 versus control using one-way ANOVA for repeated measurements and Dunnet’s test.

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Molecular Medicine

ISSN: 1528-3658