Hypoxia May Increase Rat Insulin mRNA Levels by Promoting Binding of the Polypyrimidine Tract-binding Protein (PTB) to the Pyrimidine-rich Insulin mRNA 3′-Untranslated Region

Table 1 The sequences of the different ins-PRS probes used and comparison to the TH 3′-UTR. Ins-PRS, insulin pyrimidine-rich sequence; TH HIPBS, tyrosine hydroxylase hypoxia inducible protein binding sequence. The core binding sequences for respective mRNA are marked in bold and mutated nucleotides are underlined.

mRNA probe	Sequence
TH HIPBS	5′CUUUCCCAAAGUCUCCAUCCCCUUCUCCAACCUUUCCU^a
Ins-PRS wild-type	5′UCCACCACUCCCCGCCCACCCCUCU^b
Ins-PRS mutant 1	5′UCCACCACUCCCCGCCCACCACUCU
Ins-PRS mutant 2	5′UCCACCACUCCCCUCCCACCCCUCU

^aFrom Gene Bank accession number M10244, 5′ is at position 1540, HIPBS is located between 1552 and 1579 (46).
^bFrom Gene Bank accession number J00747, 5′ is at position 4516.

ISSN: 1528-3658