Northern blot analysis of GRP78, ERp72, p58IPK, CHOP10, and Clusterin mRNAs in antropyloric mouse tissues. Each lane contained 10 µg of total RNA. Lanes 1 and 3, RNA samples from normal tissues from 8-week-old and 1-year-old wild-type (TFF1+/+) mice, respectively. Lanes 2 and 4, RNA samples from antropyloric tumors from 8-week-old and 1-year-old null (TFF1−/−) mice, respectively. Hybridization was carried out using 32P cDNA probes. The GAPDH probe was used as a positive internal loading control. The TFF1 probe was used as control of TFF1 null mutation.