Fig. 4

PARP inhibition by PJ34 inhibits NF-κB activation in immunostimulated macrophages. (A) PJ-34 pretreatment (30 µM) inhibits LPS-induced NF-κB DNA binding in J774 cells. Nuclear extracts were prepared 45 min after LPS (10 µg/ml) challenge and NF-κB DNA binding was assessed using the gel mobility shift assay. (B) Luciferase activity in RAW macrophages transiently transfected with an NF-κB promoter construct. PJ-34 pretreatment (30 µM) inhibited LPS-induced increases in luciferase activity. Data represent mean ± SEM values of n = 9 wells from three experimental determinations. *p < 0.05 represents significant increase in luciferase activity after LPS and in #p < 0.05 represents significant inhibition by PJ34.