Fig. 3

Microglia obtained from an SIV-infected monkey produce iNOS following 24-hr LPS stimulation

cDNA was made from total cellular RNA isolated from microglia (monkey 262) stimulated with LPS (+) or cultured in medium alone (−). PCR was performed with primers specific for iNOS or L32. Samples to which reverse transcriptase was (+RT) or was not (−RT) added are indicated. The expected PCR product for iNOS was obtained in microglia treated with LPS. The expected size PCR products for L32 were obtained, and the data indicate that equal amounts of cDNA were used for the PCR reaction.