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Fig. 2 | Molecular Medicine

Fig. 2

From: Role of IgE Immune Complexes in the Regulation of HIV-1 Replication and Increased Cell Death of Infected U1 Monocytes: Involvement of CD23/FcεRII-Mediated Nitric Oxide and Cyclic AMP Pathways

Fig. 2

Effect of IgE-IC on HIV mRNA accumulation in U1 low cells

RNA from U1 cultured for 48 hr was isolated and 1 µg of RNA was amplified as described in Materials and Methods. The oligomer hybridization autoradiography demonstrates the presence of amplified HIV-1 gag (Panel A, A-D; Panel B, A-E) and GAPDH-RNA (Panel A, E-H; Panel B, F-J). (A) Lane A, no RNA; Lane B, U1; Lane C, U1 + IL-4; Lane D, U1 + IL-4/IgE-IC; Lane E, U1; Lane F, U1 + IL-4; Lane G, U1 + IL-4/IgE-IC (1µg anti-IgE); Lane H, U1 + IL-4/IgE-IC (2 µg anti-IgE). (B) Lane A, U1 + IL-13; Lane B, U1 + IL-13/IgE-IC; Lane C, U1 + IL-13 + IL-4/IgE-IC; Lane D, same as Lane C with no RT; Lane E, THP-1 productively infected with HIV; Lane F, U1 + IL-13; Lane G, U1 + IL-13/IgE-IC; Lane H, U1 + IL-13 + IL-4/IgE-IC; Lane I, same as Lane H with no RT; Lane J, THP-1 productively infected with HIV.

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