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Fig. 6 | Molecular Medicine

Fig. 6

From: Role of IgE Immune Complexes in the Regulation of HIV-1 Replication and Increased Cell Death of Infected U1 Monocytes: Involvement of CD23/FcεRII-Mediated Nitric Oxide and Cyclic AMP Pathways

Fig. 6

Effects of IgE-IC and CD23-ligation on p24 levels and cell numbers in U1 high cells

The cells were preincubated for 48 hr in medium alone or with IL-4 (10 ng/ml), then washed and postincubated (2 × 105/ml) with IgE (10µg/ml) for 1 hr before the addition of anti-IgE (10 µg/ml). Some IL-4-treated cells were also incubated with CD23-mAb or CD19-mAb (20 µg/ml) for 1 hr before the addition of anti-MIg (10 µg/ml). Following 48 hr, p24 levels and the number of cells were evaluated. Shown are mean ± SD values from three distinct U1high cell experiments.

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Molecular Medicine

ISSN: 1528-3658