Fig. 4From: Reduction of Food Intake and Weight Gain by the ob Protein Requires a Specific Secondary Structure and Is Reversible Intramolecular disulfide linkage in ob protein SDS-PAGE of recombinant mouse ob proteins in the presence (Lanes 1 to 3) and absence (Lanes 4 to 6) of DTT. (Lanes 1 and 4) ob protein purified from insect cells. (Lanes 2 and 5) Wild-type ob protein purified from bacteria. (Lanes 3 and 6) Mutant ob-C167A protein purified from bacteria.Back to article page