Reduction of Food Intake and Weight Gain by the ob Protein Requires a Specific Secondary Structure and Is Reversible

Table 1 Effect of ob proteins on body weights of ob/ob mice

		ob Protein (mg/kg/day)
		Bacterially Expressed		Baculovirus Expressed (SF9 cells)
	Saline	0.3	3.0	0.3	3.0
Day 0	26.2 ± 1.94	27.6 ± 1.85	26.4 ± 3.14	27.2 ± 1.33	28.0 ± 2.53
Day 5	31.0 ± 1.26	30.2 ± 1.94	28.8 ± 2.79	29.0 ± 0.63	29.6 ± 2.24
Day 10	35.0 ± 1.41^a	32.8 ± 2.14	29.2 ± 2.93^a	30.6 ± 1.10	29.6 ± 1.85^a
Δ weight gain	8.8	5.2	2.8	3.4	1.6

Groups of five ob/ob mice were administered daily with saline or ob protein at different concentrations.
^aChange in body weight in grams between control and treated animals at Day 10 was significantly different (P < 0.01) as determined by a nonparametric Wilcoxon test (unpaired; two-tailed).

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