Skip to main content

Table 1 PCR primers and conditions for microsatellite polymorphisms

From: Prenatal Diagnosis for Recessive Dystrophic Epidermolysis Bullosa in 10 Families by Mutation and Haplotype Analysis in the Type VII Collagen Gene (COL7A1)

       Method of Detection
Locus Symbol Reported Localization Primer Sequences Annealing Temperature (°C) Fragment Size (bp) Heterozygosity Acrylamide Gel Enzyme Digestion
D3S1029 3p21.31-p21.2 L 5′-ATACTCTGGAC CCAGATTGATTAC-3′ R 5′-TAATTCCCAAATGGTTTAGGGGAG-3′ 60 168 0.62 +
APEH 3p21.3-p21.2 L 5′-GAGTCCATGGCAGCAGTTGGCAACCATGGC-3′ R 5′-TAGATCTGAGCCCGGTGCCTGGGCCACGAA-3′ 60 535/383,152 0.48 HindIII
D3S1235a 3p21.3-p21.2 L 5′-GACAGAGTCAGACTCCATCT-3′ R 5′-CTTCCCATTTTCAATCCCTAG-3′ 60 133–137 0.44 +
D3S1573a 3p21.2-pl4.2 L 5′-CCAGTAAATCACAGGGCTAT-3′ R 5′-ITCATTTTTGCTTATTAAGATATGC-3′ 57 136–154 0.79 +
D3S1581b 3p21.2-pl4.2 L 5′-ACAGAACTGCCAAACCATCC-3′ R 5′-CCTGGGTCTTTATGCTGCAA-3′ 59 245 0.88 +
D3S2 3p21.2-p21.1 L 5′-ACTATCTTGCCCTAGAGCAA-3′ R 5′-CCAAGTGGCAGAGCTACTTA-3′ 55 473/236,237 0.38 MspI
D3S1076 3p21.2-p21.1 L 5′-ATTCCTGCATATGATCCAACTG-3′ R 5′-ACTAAGAGCAATCACTTAACTTAG-3′ 60 95 0.59 +
  1. aLocated on the same YAC.
  2. bLocated within 70 kb of the COL7A1 locus.