Table 2 PCR primers and conditions for intragenic (COL7A1) polymorphisms
Method of Detection | |||||||
|---|---|---|---|---|---|---|---|
Intragenic Location | Primer Sequences | Annealing Temperature (°C) | Fragment Size (bp) | Heterozygosity | Heteroduplex | Enzyme Digestion | |
nt 1787 | L 5′-AGGAGGCTTGTGGAGGACAG-3′ | 60 | 195/101,59 | 0.29 | + | or | AluI |
Exon 14 | R 5′-CACTGCCTGTGCTCCAGCTA-3′ | ||||||
nt 2820 | L 5′-CCTCCCTGATTCCTGAGCTT-3′ | 55 | 697/592,105 | 0.36 | + | or | PvuII |
Exon 21 | R 5′-GGAGGAGTCACTCAGAGTCG-3′ | ||||||
Exons | L 5′-AGATCTCTCCCTTCTGGGTT-3′ | 55 | 682 | n/d | + | − | |
22–24 | R 5′-GGAGAGGTAGAATCTGGCTG-3′ | ||||||
Exons | L 5′-CCAGGAGAGTGAGGGAAGAG-3′ | 57 | 581 | n/d | + | − | |
74–76 | R 5′-CAAGTCAGTCCCTAGTGCCA-3′ | ||||||
Exons | L 5′-CGGGCTCGTTGTATTCTAAG-3′ | 55 | 513 | n/d | + | − | |
103–104 | R 5′-CAAAAGCTACCACACTGGTG-3′ | ||||||