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Table 2 PCR primers and conditions for intragenic (COL7A1) polymorphisms

From: Prenatal Diagnosis for Recessive Dystrophic Epidermolysis Bullosa in 10 Families by Mutation and Haplotype Analysis in the Type VII Collagen Gene (COL7A1)

      Method of Detection
Intragenic Location Primer Sequences Annealing Temperature (°C) Fragment Size (bp) Heterozygosity Heteroduplex Enzyme Digestion
nt 1787 L 5′-AGGAGGCTTGTGGAGGACAG-3′ 60 195/101,59 0.29 + or AluI
Exon 14 R 5′-CACTGCCTGTGCTCCAGCTA-3′       
nt 2820 L 5′-CCTCCCTGATTCCTGAGCTT-3′ 55 697/592,105 0.36 + or PvuII
Exon 21 R 5′-GGAGGAGTCACTCAGAGTCG-3′       
Exons L 5′-AGATCTCTCCCTTCTGGGTT-3′ 55 682 n/d +  
22–24 R 5′-GGAGAGGTAGAATCTGGCTG-3′       
Exons L 5′-CCAGGAGAGTGAGGGAAGAG-3′ 57 581 n/d +  
74–76 R 5′-CAAGTCAGTCCCTAGTGCCA-3′       
Exons L 5′-CGGGCTCGTTGTATTCTAAG-3′ 55 513 n/d +  
103–104 R 5′-CAAAAGCTACCACACTGGTG-3′