Fig. 3

Northern blot analysis of hCsx mRNA

The upper panel shows a human multiple tissue northern blot containing 2 µg of poly A⁺ RNA from eight different adult tissues probed with a 5′ portion of the hCsx cDNA (nucleotides 1–608, Probe C, Fig. 2B). Hybridization signals were seen only in the heart lane. Hybridization of the blot with a PstI fragment containing the entire coding region and a part of the 3′ untranslated region of the rat glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is shown in the lower panel to verify RNA integrity (23). Unequal loading of the lanes is noted with more intense bands in the placenta and pancreas lanes.