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Fig. 4 | Molecular Medicine

Fig. 4

From: Molecular Cloning, Chromosomal Mapping, and Characterization of the Human Cardiac-Specific Homeobox Gene hCsx

Fig. 4

Expression of hCsx mRNA in human fetal heart and different pathologic states

(A) Representative composite autoradiogram showing expression of hCsx mRNA in human myocardium from selected fetal stages and several pathologic states. Total RNA (5 µg) was used for each lane and ribonuclease protection analysis was performed using hCsx and gamma-actin antisense riboprobes. Yeast tRNA (10 µg) was used as a negative control. The left panel shows the undigested probes, hCsx, 523 nucleotides (nt) in length and γ-actin, 145 nt in length. Protected fragments of the expected sizes (hCsx, 428 nt; γ-actin, 140 nt) were present in all samples examined. (B) Densitometric analysis of hCsx transcript levels during human embryonic development and in different disease states of the LV myocardium. The relative abundance of the hCsx mRNA was normalized to the level of γ-actin mRNA in each sample. Densitometric score of the normal control was defined as 1.0. Data shown represents the mean ± SEM. LV, left ventricle; WK, week; NRML, normal; DCM, dilated cardiomyopathy (n = 6); ICM, ischemic cardiomyopathy (n = 6); CHD, congenital heart disease (n = 2).

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