Fig. 3

Toxicity and DNA fragmentation initiated by TNF or anti-CD95 in hepatocyte cultures from wt or lpr MRL/MpJ mice

(A) Hepatocytes were incubated with or without 333 nM ActD and with various concentrations of anti-CD95. Viability was measured after 20 hr by quantitation of formazan forming capacity. Neutralizing anti-TNF antibody (αTNF) was added at a dilution of 1:200. (B) Hepatocytes were incubated with 333 nM ActD and various concentrations of TNF before toxicity was determined after 20 hr. All data are means ± SD, n = 3. (C) Hepatocytes were incubated for 10 hr before they were lysed for DNA extraction. DNA was analyzed on 1% agarose gels and stained with ethidium bromide. (Lane 1) control-lpr. (Lane 2) control-wt. (Lane 3) ActD-lpr. (Lane 4) ActD-wt. (Lane 5) ActD/TNF (100 ng/ml)-wt (Lane 6) ActD/TNF-lpr. M, n × 123 bp molecular weight marker.