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Fig. 5 | Molecular Medicine

Fig. 5

From: The 55-kD Tumor Necrosis Factor Receptor and CD95 Independently Signal Murine Hepatocyte Apoptosis and Subsequent Liver Failure

Fig. 5

Toxicity initiated by combinations of TNF and anti-CD95 in hepatocyte cultures from wt or tnf-r1° C57B1/6 mice

(A) Hepatocytes were incubated for 20 hr with different combinations of TNF and anti-CD95. (B) Hepatocytes were incubated for 20 hr with anti-CD95 alone or in combination with 2 µg/ml TNF or 1:200 neutralizing anti-TNF antibody (anti-TNF). (C) Hepatocytes were sensitized with 5 mM GalN or 333 nM ActD and then incubated with anti-CD95 for 20 hr. Neutralizing anti-TNF antibody (αTNF) was used at a 1:200 dilution. All data are means ± SD from triplicate determinations.

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