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Fig. 7 | Molecular Medicine

Fig. 7

From: The 55-kD Tumor Necrosis Factor Receptor and CD95 Independently Signal Murine Hepatocyte Apoptosis and Subsequent Liver Failure

Fig. 7

Comparison of apoptosis, DNA fragmentation, and cell lysis in livers from wt or tnf-r1° C57Bl/6 mice

(A) Six mice from each substrain were injected intravenously with 2 µg/mouse anti-CD95. After 5 or 8 hr three animals from each substrain were terminally anesthesized. Blood was withdrawn into heparinized syringes for the determination of ALT and sorbitol dehydrogenase (SDH), and livers were excised for the determination of DNA fragmentation. Three untreated control mice were killed after the same time periods as a control for hepatic DNA fragmentation. They had normal levels of ALT (70 U/l) or SDH (15 U/l). (B) Histological evidence of apoptosis and severe hemorrhage 5 hr after the injection of anti-CD95. The specimen was stained with hematoxilin/eosin and photographed at an initial magnification of 400×. Numerous apoptotic bodies and cells with strongly marginated and condensed chromatin are seen. (C) Liver tissue 5 hr after injection of anti-CD95, photographed at an original magnification of 630×. Apoptotic hepatocyte death occurs in the absence of infiltrating inflammatory cells.

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