The 55-kD Tumor Necrosis Factor Receptor and CD95 Independently Signal Murine Hepatocyte Apoptosis and Subsequent Liver Failure

Table 1 Anti-CD95-induced DNA fragmentation in hepatocytes from wt or tnf-r1° C57Bl/6 mice

	Hepatocyte DNA-Fragmentation (% of saline-treated control ± SD)^a
	No Pretreatment		Pretreatment with 5 mM GalN
Mouse Substrain	Saline	Anti-CD95	Saline	Anti-CD95
wt mice	100 ± 27	330 ± 23	117 ± 21	340 ± 29
tnf-r1° mice	100 ± 16	570 ± 34	95 ± 2	540 ± 19

^aEighty thousand hepatocytes plated in 24-well plates in 200 µl of medium were pretreated with 5 mM GalN or saline for 30 min, before they were challenged with 100 ng/ml anti-CD95 or solvent control. After 6 hr, cells were lysed. Low molecular weight DNA was prepared by centrifugation and subsequently analysed in an ELISA determining the amount of cytosolic oligonucleosomal DNA.

ISSN: 1528-3658