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Fig. 4 | Molecular Medicine

Fig. 4

From: Retroviral Vector Design for Gene Therapy of Cancer: Specific Inhibition and Tagging of BCR-ABLp190 Cells

Fig. 4

Specific suppression of BCR-ABL p190 mRNA transcripts in selected Ba/F3+p190 cells infected with the antisense virus

Total RNA was isolated from Ba/F3+p190 cells (Lane 1), selected Ba/F3+p190 cells infected with the antisense virus (Lane 2), and selected Ba/F3+p190 cells infected with the sense virus (Lane 3). Cellular RNA was hybridized to a human ABL probe (top) and to a mouse β-actin cDNA (bottom). Autoradiography was for 9 hr at − 70°C.

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