Early growth phase response in
Autoradiograms show 2-D gel profiles of metabolically labelled proteins in aliquots of a 3-day M. tuberculosis H37Rv culture in 7H9+ADc medium without any addition (A) and with 300 µM menadione, a superoxide generating redox cycling compound (B). Circles indicate induced polypeptides and numbers indicate their apparent molecular weights. K, DnaK (Hsp70) (26); L, GroEL (Hsp65) (26,35). DnaK is also up-regulated by menadione.