Fig. 6
From: Response of Mycobacterium tuberculosis to Reactive Oxygen and Nitrogen Intermediates
M. tuberculosis response to cumene hydroperoxide
(A) SDS-PAGE analysis of metabolically labelled polypeptides in aliquots from culture grown in 7H9+ADC medium exposed to increasing amounts of cumene hydroperoxide. Lanes: 1, 37°C control; 2–4, 100 µM; 200 µM; and 500 µM cumene hydroperoxide. (B) Analysis by 2-D gel electrophoresis of the 500 µM cumene hydroperoxide treated sample (Panel A, Lane 4). Two major induced polypeptides are labeled as heat shock proteins 15 and 28 on the basis of their positions and earlier published data (26). The 45- and 53-kD polypeptides are referred to in the text as Chp45 and Chp53. DnaK and GroEL (Hsp70 and Hsp65) are indicated as markers to facilitate comparisons.