Table 1 Purification scheme for the protein catalyzing the conversion of D -dopachrome into DHICA
Step | Total protein (mg) | Enzyme Activity (µmol DHICA min−1) | Specific Activity (µmmol DHICA min−1 mg−1) | Yield (%) |
|---|---|---|---|---|
Homogenate | 6980 | 7.5 | 0.001 | 100 |
High-speed supernatant | 3770 | 6.6 | 0.002 | 88 |
Phenyl-sepharose | 200 | 2.9 | 0.014 | 39 |
Mono S | 0.90 | 1.6 | 1.8 | 22 |
Superdex | 0.28 | 1.1 | 4.0 | 14 |
SDS-PAGE | 0.10 | — | — | — |