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Table 1 Effects of antioxidants on edema toxin and lethal toxin actions in RAW264.7 M ϕ cultures

From: Role of Macrophage Oxidative Burst in the Action of Anthrax Lethal Toxin

   Percent of Initial Toxin Activity
Antioxidant Concentration EdTxa LeTxb
None 100% 100%
DTT 10 mM 91 3
BME 10 mM 94 6
EtOH 10%(v/v) 99 12
DMSO 10%(v/v) 108 7
ascorbate 100 mM 95 21
α-tocopherol 100 mM 90 18
mepacrine 100 mM 88 10
NACc 50 mM 99 5
metc 100 mM 103 9
  1. aEdTx-induced increase of cellular cAMP was determined by radioimmunoassay, using a cAMP scintillation proximity assay. 100% activity is defined as the amount of cAMP in cells (950 fmol/µg protein) 45 min after addition of toxin (0.1 µg PA + 0.1 µg EF/ml), after normalization for cAMP present in untreated cells.
  2. bLeTx-induced cytotoxicity was measured by release of 51Cr from prelabeled cells (see Fig. 1 legend).
  3. cNAC and met were preincubated with cells for 16 hr before toxin challenge (see Fig. 3).