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Table 2 Correlation of M ϕ sensitivity to LeTx with ability to mount an oxidative burst

From: Role of Macrophage Oxidative Burst in the Action of Anthrax Lethal Toxin

  PMA-Induced O2 Productiona LeTx-Cytotoxicityb
  (nmol/107 cells/hr) (Relative Activity) (% of Maximal Release) (Relative Activity)
A. Murine Lines     
J774 21 100% 60 100%
J774.16 34 162 69 115
3C3 14 67 52 87
D-9 11 52 45 75
RAW264.7 75 357 79 134
IC -21 <1 <5 0 0
B. 1° Human Monocytes     
LN (CGD Patient) 0 0 <1 <2
PH (Control) 23 109 44 73
  1. aSuperoxide anion production was determined by cytochrome c reduction assay after addition of 10 ng/ml PMA, as described in Fig. 1.
  2. bCytotoxicity was assayed by 51Cr-release, as described in Fig. 1. Relative activities are compared using values derived from J774 Mϕ line as the 100% standard (see text).