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Fig. 2 | Molecular Medicine

Fig. 2

From: Pentoxifylline and Other Protein Kinase C Inhibitors Down-Regulate HIV-LTR NF-κB Induced Gene Expression

Fig. 2

Specificity of the DNA-protein interaction involving a 32P-labeled oligonucleotide containing NF-κB motif and active NF-κB in a cell-free system

The specificity of the interaction of active NF-κB with 32P-oligonucleotide in the PKC- or PKA-catalyzed cell-free activation system was determined by competition experiments with nonradioactive oligonucleotides carrying the wild-type (A) or mutated (B) NF-κB motif (described in Materials and Methods). The cell-free NF-κB activation system was as described in Fig. 1 with 1 µg of fractionated cytoplasmic protein, 20,000 cpm of 32P-labeled oligonucleotide, 0.2 U of PKC (designated by E) plus the indicated amounts (ng/5 µl reaction mixture) of nonradioactive wild-type or mutant oligonucleotides.

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