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Table 1 Peptides from urease β-subunit and L23 protein and binding affinity to HLA-B27

From: The Evolutionarily Conserved Ribosomal Protein L23 and the Cationic Urease β-Subunit of Yersinia enterocolitica O:3 Belong to the Immunodominant Antigens in Yersinia-Triggered Reactive Arthritis: Implications for Autoimmunity

Position Within Protein

Sequencea

Binding Affinityb

U β-[2129]

N

R

G

T

K

S

S

A

G

U β-[60–68]

V

R

N

T

G

D

R

P

I

+

U β-[65–73]

D

R

P

I

Q

V

G

S

H

U β-[80–88]

N

R

A

L

E

F

D

R

A

U β-[86–94]

D

R

A

A

A

Y

G

K

R

U β-[93–101]

K

R

L

N

I

S

S

T

T

+

U β-[103–lll]

I

R

F

E

P

G

D

E

T

+

U β-[144–152]

E

R

P

D

K

L

E

A

I

U β-[152–160]

I

R

R

A

A

E

R

G

F

U β-[153–161]

R

R

A

A

E

R

G

F

K

+ +

-

-

-

-

-

-

-

-

-

L23-[2–10]

I

R

E

E

R

L

L

K

V

L23-[5–13]

E

R

L

L

K

V

L

R

A

L23-[ll–19]

L

R

A

P

H

V

S

E

K

+

L23-[67–75]

K

R

H

G

Q

R

V

G

R

+

L23-[71–79]

Q

R

V

G

R

R

S

D

W

L23-[74–82]

G

R

R

S

D

W

K

K

A

L23-[75–83]

R

R

S

D

W

K

K

A

Y

+

  1. aSingle letter code is used; R at P2 as dominant anchor is given in bold and underlined.
  2. bRelative binding affinities were determined as examplified in Fig. 4: (−) nonbinders; optical band density/mm2 greater than ×2 the band density of untreated control. (+), low to intermediate binders; band density greater than ×2, greater than ×4 the untreated control. (+ +) strong binder; band density greater than ×4 the untreated control.