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Fig. 2 | Molecular Medicine

Fig. 2

From: X Chromosome Inactivation Patterns Correlate with Fetal-Placental Anatomy in Monozygotic Twin Pairs: Implications for Immune Relatedness and Concordance for Autoimmunity

Fig. 2

Flow diagram of the HpaII/PCR assay

After radioactive PCR across the triplet (CAG) repeat of the AR gene, two alleles of different length are observed in mock digested genomic DNA from heterozygous females. When the PCR is performed after the HpaII digestion of the target DNA, the intensity of each band reflects the relative degree of methylation of each AR allele. A schematic illustration of the results obtained for various degrees of inactivation (methylation) of the X chromosome bearing the lower AR allele is shown at the bottom of the figure.

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