Time-dependent appearance of fibrocytes in subcutaneously implanted wound chambers
Cellular analysis was performed on 5–10 µl of fluid aspirated from wound chambers at the intervals shown. Cells were enumerated by cytofluorimetry and then double-stained with rhodamine-conjugated monoclonal anti-murine CD34 plus FITC-conjugated monoclonal antimurine collagen I. Similar results were obtained by double staining with anti-CD34 plus antivimentin antibodies. Each time point represents the mean ± SEM of three or four independent fluid samplings.