Immunohistochemical analysis of murine fibrocytes in wound chambers and cutaneous scar tissue.
(a) Representative sections obtained from wound chambers 28 days after implantation in mice (400×). Adjacent sections were stained with either monoclonal anti-murine CD34 (left) or with a isotypic antibody control (right) and developed with an immunoperoxidase-linked secondary antibody. The areas of homogenous blue staining are polyvinylalcohol sponge. Qualitatively similar results were obtained in wound chambers removed 7, 14, and 56 days after implantation. However, progressively more fibrosis (and anti-CD34 staining) was observed with increasing time after implantation. (b) Immunohistochemical analysis of a mouse cutaneous scar obtained 28 days after an experimentally induced cutaneous incision (400×). Sections were stained as above with either monoclonal anti-murine CD 34 (left) or with an isotypic antibody control (right) and developed with an immunoperoxidase-linked secondary antibody. Sections obtained from corresponding regions of control, nonscarred mouse skin showed no detectable staining with anti-CD34 antibody.