In vivo γδ T Cell Priming to Mycobacterial Antigens by Primary Mycobacterium tuberculosis Infection and Exposure to Nonpeptidic Ligands

Table 1 Vγ9/Vδ2 responses to different phosphoantigens in PPD skin test-positive children, age-matched PPD-negative controls, and healthy rhesus monkeys

		Vγ9/Vδ2 expansion index after in vitro culture with
PBMC	(n)	Rib-1-P	Xyl-1-P	DMAPP	TUBAg	IPP	DPG	MEP
Healthy PPD⁻children	16	7 ± 6	2 ± 1	5 ± 2	6 ± 2	6 ± 1	n.d.	n.d.
MTB-infected PPD⁺ children^a	22	18 ± 7	30 ± 14	54 ± 24	50 ± 14	61 ± 20	n.d.	n.d.
Healthy rhesus monkeys	12	n.d.	n.d.	n.d.	8 ± 2	8 ± 3	6 ± 2	9 ± 3

PBMC were stimulated for 10–14 days with nonpeptidic antigens as described in Materials and Methods. The expansion index was calculated as the absolute number of Vδ2 T cells in stimulated cultures divided by the absolute number of Vδ2 T cells in the initial cultures prior to the antigenic exposure, n.d., not determined.
^ap < 0.01 when compared to the healthy PPD⁻ group.

ISSN: 1528-3658