Fig. 1

Expression of GATA-4 and GATA-6 mRNA during adrenal development and in vivo manipulation of adrenal function. H&E (A, C) and dark field views (B, D) of in situ hybridization demonstrating expression of GATA-6 mRNA in the adrenal cortex at postnatal day 25 (A, B) but not of GATA-4 mRNA (C, D). c, adrenal cortex; m, adrenal medulla. Original magnification (×200), bar = 50 µm. (E) RNase protection assay showing expression of GATA-6 mRNA in normal adrenal cortex (Co). No change in the expression level was noted in animals treated with ACTH or short or long treatment with dexamethasone (short- and long dexam., respectively). No GATA-4 mRNA could be detected in the adrenals of control or treated animals. Mouse testis RNA was used as a positive control.