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Fig. 3 | Molecular Medicine

Fig. 3

From: IL-6 is associated to IGF-1Ec upregulation and Ec peptide secretion, from prostate tumors

Fig. 3

Determination of the effect of IL-6 on the IGF-1Ec expression. a: Co-incubation of prostate cancer cell lines with the cells of the innate or adaptive immune system (IIR: innate immune response, SL: sensitized lymphocytes) led to JAK2/STAT3 pathway activation as assessed by western blot analysis. b: The exogenous administration of 50 nM of IL-6 for 1 h in both prostate cancer cell lines was associated with a significant increase of IGF-1Ec expression in both cell lines (qRT-PCR). This effect was abolished with the addition of anti-IL-6 or anti-IL-6R antibodies. c: Treatment of PC-3 and DU-145 cells with IL-6 led to the induction of JAK2 and STAT3 phosphorylation as assessed by western blot analysis. This effect was reversed when these cell lines were treated with the anti-IL-6R antibody. d: In both cell lines the IGF-1Ec upregulation resulted after their exposure to the IR cells (innate or adaptive) was reversed when treated with anti-IL-6R antibody (qRT-PCR). e: Effect of the anti-IL-6R antibody on the JAK2 and STAT3 phosphorylation induced by the immune system attack (innate or adaptive) on PC-3 cells (western blot analysis). Antibody treatment led to a significant reduction in the phosporylation status of JAK2 and STAT3. Similar results were observed for the DU-145 cells. F: Both prostate cancer cell lines under the attack of the IR (innate or adaptive) were found to express significant amounts of IL-6 (qRT-PCR) compared to the controls. Conditioned media collected from the co-culturing experiments had the same effect on IL-6 expression when introduced in wt prostate cancer cell lines. The IL-6 upregulation was reversed when the cells were treated with the anti IL-6R antibody (**: p < 0.005, ***:p < 0.0005)

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