Fig. 4

Inhibition of NLRP3 activation by EP is independent of lysosomal rupture. Peritoneal mouse macrophages were primed with ultra-pure LPS (1 μg/ml) for 3 h. in the presence or the absence of 5 mM EP, and then stained with acridine orange (1 μg/ml) for 30 min at 37 °C. Cells were then stimulated by alum (20 μg/ml) or silica (10 μg/ml) 6h. a After stimulation, cells were analyzed by flow cytometry for lysosomal acridine orange fluorescence. b Cytotoxicity was assessed using LDH assay. Results are means ± SEM (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001