Fig. 6
From: Stachydrine protects eNOS uncoupling and ameliorates endothelial dysfunction induced by homocysteine
STA increased the expressions of GTPCH1 and DHFR via Nrf2 activation. Western blotting analysis of Nrf2 protein levels in whole (a), nuclear (b) and cytoplasmic (c) fractions. Quantification of Nrf2 protein levels as in (a, b and c (bottom)). β-actin, Histone H3 and GAPDH were used for internal controls in whole, nuclear and cytoplasmic fractions, respectively. d ECs were transfected with scrambled siRNA or Nrf2 siRNA for 24 h and then treated with STA (10 μM) for another 24 h. The mRNA levels of Nrf2, GTPCH1 and DHFR were assessed by using qRT-PCR. e Western blotting analysis of Nrf2, GTPCH1 and DHFR protein levels. Quantification of Nrf2, GTPCH1 and DHFR protein levels as in H (right, n = 3). Data shown were mean ± SEM. *P < 0.05