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Fig. 4 | Molecular Medicine

Fig. 4

From: Targeting DEC-205DCIR2+ dendritic cells promotes immunological tolerance in proteolipid protein-induced experimental autoimmune encephalomyelitis

Fig. 4

Effect of immunization with αDEC-205-HA109–117 on populations of CD4+CD25+Foxp3+ Treg cells (HA109–117 abbreviated in the figure as HA due to space and readability concerns). Small numbers of TCR transgenic CD4 + CD25 + Foxp3+ Treg cells that recognize an HA peptide were injected into immunocompetent congenic recipients, which were subsequently injected with either αDEC-205-HA109–117 or αDCIR2-HA109–117 fusion mAbs. a FACS plot of Foxp3 (GFP), CD25 and CD4 expression in cells isolated from spleens, mesenchymal (mLN), and supraclavicular (scLN) lymph nodes of mice treated with αDEC-205-HA109–117 mAb. b FACS plot of Foxp3 (GFP), CD25 and CD4 expression in cells isolated from spleens, and mesenchymal and supraclavicular lymph nodes of control mice. In untreated mice, congenic marker positive Treg cells that can be tracked in peripheral lymphoid tissues maintain Foxp3 and CD25 expression but do not undergo proliferation (day 6 after injection). c FACS plot of Foxp3 (GFP), CD25 and CD4 expression in cells isolated from spleens and mesenchymal and supraclavicular lymph nodes of mice treated with αDCIR2-HA109–117 mAb. d Histogram of results from analyses shown in (a-c). Both αDEC-205-HA109–117 and αDCIR2-HA109–117 fusion mAbs promote proliferative expansion of antigen-specific Treg cells. Increases in the number of Foxp3-GFP+ Treg cells is observed after treatment with αDCIR2-HA109–117 fusion antibodies

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