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Fig. 2 | Molecular Medicine

Fig. 2

From: Ligation of free HMGB1 to TLR2 in the absence of ligand is negatively regulated by the C-terminal tail domain

Fig. 2

Pure C-tail truncated HMGB1 (Δ30) does not mediate cytokine production in vitro. HMGB1 and Δ30 were added at the indicated concentrations to a) HEK cells transfected with functional TLR 2, 1/2 and 2/6 dimers, b) whole blood collected from healthy volunteers and c) alveolar macrophage cells. Serum cytokine levels were quantified by ELISA after 24 h. PGN (2.5 μg/mL) and Pam3CSK4 (2.5 μg/mL), known TLR2 ligands, were included as positive controls. LPS (100 ng/mL) was included as a TLR4 positive control. (a = representative data from 1/3 experiments; b = combined data from 5 individual donors; c = 1 experiment performed in triplicate wells)

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