Fig. 2
SPR analyses of redox forms of HMGB1 binding to TLR4/MD-2 complex or TLR4. a-c TLR4/MD-2 complex was coated on the CM5 chip; disulfide HMGB1 binds to complex with a KD of 0.42 ± 0.01 μM; reduced HMGB1 binds with a KD of 3.93 ± 0.01 μM; HMGB1 3S mutant binds with a KD of 3.02 ± 0.02 μM. d-f TLR4 was coated on the chip; HMGB1 binds to TLR4 with a KD of 0.64 ± 0.01 μM; reduced HMGB1 binds with a KD of 0.65 ± 0.01 μM; HMGB1 3S mutant binds with a KD of 4.20 ± 0.09 μM. Data are representative of three repeats