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Fig. 3 | Molecular Medicine

Fig. 3

From: Exploring the biological functional mechanism of the HMGB1/TLR4/MD-2 complex by surface plasmon resonance

Fig. 3

SPR analyses of GST-A-box binding to TLR4/MD-2, TLR4 and MD-2. a TLR4/MD-2 complex was coated on the CM5 chip, GST-A-box binds to complex with a KD of 1.21 ± 0.02 μM. b TLR4 was coated on the chip, GST-A-box binds to TLR4 with a KD of 0.59 ± 0.01 μM. c GST-A-box was coated on the CM5 chips, MD-2 was used as analyte, MD-2 binds to GST-A-box with a KD of 3.25 ± 0.17 μM. GST tag was used as negative control. Data are representative of three repeats

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