Fig. 4
SPR analyses of GST-B-box binding to TLR4/MD-2, TLR4 and MD-2. a TLR4/MD-2 complex was coated on the CM5 chip, GST-B-box binds to TLR4/MD-2 complex with a KD of 5.78 ± 0.22 μM. b TLR4 was coated on the chip, GST-B-box has no significant binding to TLR4. c GST-B-box was coated on the CM5 chips, MD-2 was used as analyte, MD-2 showed better binding affinity to GST-B-box (KD of 1.41 ± 0.03 μM) relative to GST-A-box (KD = 3.25 ± 0.17 μM). GST tag was used as negative control. Data are representative of three repeats