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Fig. 5 | Molecular Medicine

Fig. 5

From: Exploring the biological functional mechanism of the HMGB1/TLR4/MD-2 complex by surface plasmon resonance

Fig. 5

Ternary complex formation among TLR4/MD-2, TLR4, A-box and HMGB1. a Biacore sensorgram showing molecular interactions, after injecting HMGB1 (100 nM) followed immediately by HBS buffer, HMGB1 (100 nM) or TLR4/MD-2 (100 nM) or mixture of the latter (100 nM each) onto a TLR4/MD-2 sensor chip surface. b Injecting A-box (10 μM), followed immediately by A-box (0 μg/ml of HMGB1) or HMGB1 (2.5 and 5 μg/ml) onto a TLR4 sensor chip surface

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