Skip to main content

Table 1 Amino acids inserted during readthrough

From: Advances in therapeutic use of a drug-stimulated translational readthrough of premature termination codons

Basal PTC-RT
UGA UAA UAG
Arg (CGA)
(58.6 ± 2.3%)
Trp (UGG)
(41.4 ± 2.3%)
Cys (UGU/C)
-
Tyr (UAU/C)
(67.5 ± 8.9%)
Gln (CAA)
(32.4 ± 8.9%)
Tyr (UAU/C)
(73.1 + 15%)
Gln (CAG)
(25.2 ± 13.7%)
Trp (UGG)
(1.3 ± 1.4%)
Stimulated PTC-RT PTC124 (30 uM)
UGA UAA UAG
Arg (CGA)
(69.7 ± 11.3%)
Trp (UGG)
(28.8 ± 11.4%)
Cys (UGU/C)
(0.7 ± 0.7%)
Tyr (UAU/C)
(57.9 ± 11.3%)
Gln (CAA)
(39.9 ± 11.7%)
Tyr (UGG)
(43.9 ± 20.9%)
Gln (CAG)
(53.2 ± 20.3%)
Trp (UGG)
(1.8 ± 1.9%)
Stimulated PTC-RT G418 (150 uM)
UGA UAA UAG
Arg (CGA)
(64.5 ± 11.8%)
Trp (UGG)
(17.9 + 6.8%)
Cys (UGU/C)
(17.7 ± 8.0%)
Tyr (UGG)
(47.9 ± 14.1%)
Gln (CAA)
(52 ± 14.2%)
Tyr (UAU/C)
(10.8 ± 7.0%)
Gln (CAG)
(86.5 ± 8.3%)
Lys (AAG)
(2.0 ± 0.8%)
  1. The frequency of amino acids inserted during either basal or stimulated readthrough (PTC124 or G418) in the human cell line transfected with reported vectors containing different stop codons (based on Roy et al., 2016)