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Table 1 Amino acids inserted during readthrough

From: Advances in therapeutic use of a drug-stimulated translational readthrough of premature termination codons

Basal PTC-RT

UGA

UAA

UAG

Arg (CGA)

(58.6 ± 2.3%)

Trp (UGG)

(41.4 ± 2.3%)

Cys (UGU/C)

-

Tyr (UAU/C)

(67.5 ± 8.9%)

Gln (CAA)

(32.4 ± 8.9%)

Tyr (UAU/C)

(73.1 + 15%)

Gln (CAG)

(25.2 ± 13.7%)

Trp (UGG)

(1.3 ± 1.4%)

Stimulated PTC-RT PTC124 (30 uM)

UGA

UAA

UAG

Arg (CGA)

(69.7 ± 11.3%)

Trp (UGG)

(28.8 ± 11.4%)

Cys (UGU/C)

(0.7 ± 0.7%)

Tyr (UAU/C)

(57.9 ± 11.3%)

Gln (CAA)

(39.9 ± 11.7%)

Tyr (UGG)

(43.9 ± 20.9%)

Gln (CAG)

(53.2 ± 20.3%)

Trp (UGG)

(1.8 ± 1.9%)

Stimulated PTC-RT G418 (150 uM)

UGA

UAA

UAG

Arg (CGA)

(64.5 ± 11.8%)

Trp (UGG)

(17.9 + 6.8%)

Cys (UGU/C)

(17.7 ± 8.0%)

Tyr (UGG)

(47.9 ± 14.1%)

Gln (CAA)

(52 ± 14.2%)

Tyr (UAU/C)

(10.8 ± 7.0%)

Gln (CAG)

(86.5 ± 8.3%)

Lys (AAG)

(2.0 ± 0.8%)

  1. The frequency of amino acids inserted during either basal or stimulated readthrough (PTC124 or G418) in the human cell line transfected with reported vectors containing different stop codons (based on Roy et al., 2016)