Fig. 8

Panel I represents immunohistochemistry of (a) untreated carotid arteries and injured carotid arteries (b) immediately after injury (c) 2 days post injury and (d) 5 days post injury using a Leica CTR 5000 fluorescence microscope. Arteries were stained with mouse anti-HIF-1α and followed by FITC-conjugated anti-mouse secondary antibody (green stain) to visualize HIF-1α. Nuclei were stained with DAPI (Blue stain). 1 represents the lumen, 2 represents the smooth muscle cells and 3 represents the Tunica adventitia. Images shown are at 40× magnification and 63× magnification (inset images). Red arrows indicate the localization of HIF-1α expression in the smooth muscle cells. Panel II represents the staining of endothelial cells (EC) and smooth muscle cells (SMCs). The yellow arrow indicates EC layer in control (uninjured) arteries which is removed post injury and starts to regenerate 5 days post injury. Arteries were stained with rabbit anti-CD31 and mouse anti-alpha smooth muscle actin followed by Alexafluor 647 conjugated anti-rabbit secondary antibody (red stain) and FITC-conjugated anti-mouse secondary antibody (green stain) to visualize EC and SMCs respectively