Skip to main content
Fig. 2 | Molecular Medicine

Fig. 2

From: Reversal of angiotensin ll-induced β-cell dedifferentiation via inhibition of NF-κb signaling

Fig. 2

The deleterious effect of Angll is dependent on NF-κb signaling in β cells. Pancreatic β cell lines were cultured with or without Angll (1 μmol/L) in the presence or absence of sc-514, an IkB-kinase-2 inhibitor (20 μmol/L), or Irbesartan (IRB) (10 μmol/L) for 48 h. qRT-PCR analyses for (a-d) progenitor likes cell markers (Ngn3, Oct4) and (e, f) proinflammatory cytokine Il6. Data are presented as the mean ± SEM of three independent experiments (n = 6), *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA. g, h Western blotting for NGN3, OCT4, and insulin in Min6 cells and INS-1 cells. β-actin was used as a loading control. Densitometric analyses of the western blotting results are presented below. Data are presented as the mean ± SEM of three independent experiments(n = 6). **p < 0.01, ***p < 0.001 vs Control group, #p < 0.05, ##p < 0.01, ###p < 0.001 vs Vehicle group, one-way ANOVA

Back to article page