Fig. 3
From: Reversal of angiotensin ll-induced β-cell dedifferentiation via inhibition of NF-κb signaling
Angll induces the activation of NF-κb, leading to dedifferentiation and dysfunction in β cells. Pancreatic β cell lines were cultured with increasing doses of Angll for 48 h. Performing (a, b) a GSIS assay to determine (c, d) the stimulatory index in Min6 cells and INS-1 cell. qRT-PCR analyses for (e-h) progenitor like cells markers (Ngn3, Oct4), (i-l) NF-κb signaling (lκBα, p65) and (m, n) proinflammatory cytokines Il6 in Min6 cells and INS-1 cell. Data are presented as the mean ± SEM of three independent experiments (n = 6), *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA. o, p Western blotting for P65, IκBα, PDX1, NGN3, FOXO1, and the phosphorylated forms of P65 (p-P65) and IκBα (p-IκBα) in Min6 cells and INS-1 cells. β-actin was used as a loading control. Densitometric analyses of the western blotting results are presented. Data are presented as the mean ± SEM of three independent experiments. (n = 6) *p < 0.05, **p < 0.01, ***p < 0.001 vs control group, one-way ANOVA