Fig. 5
From: Reversal of angiotensin ll-induced β-cell dedifferentiation via inhibition of NF-κb signaling
(color; online only) sc-514 reversed β-cell dedifferentiation and dysfunction in db/db mice. a, c, e paraffin-embedded sections from the indicated groups were immunolabeled for insulin (green), PDX1 (red), insulin (green), NGN3 (red), insulin (green), FOXO1 (red), and DAPI (blue) (b, d, f) Quantification of labeled PDX1 or NGN3 or FOXO1 positive cells per islet. The squares (dashed white lines) show regions 9× enlarged and depicted as inserts at the top right of the corresponding images. g, h Numbers (NO.) of PDX1 or NGN3 positive cells. Scale bar (10 μm). (i) Quantification of FOXO1 translocated to the nuclei of FOXO1-positive cells. For the immunofluorescence analysis: n ≥ 4 mice per group, n ≥ 20 islets per marker. Mean ± SEM *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA