Table 1 Primer sequences for RT-qPCR
Genes | Primer sequences |
|---|---|
miR-181a | F: 5’-GGGCAGCCTTAAGAGGA-3′ |
R: 5’-CAGTGCGTGTCGTGGA-3′ | |
CRY1 | F: 5’-TCAGTTGGGAAGAAGGGATG-3′ |
R: 5’-CATTGGGGTCTGTCCTCCTA-3′ | |
TLR2 | F: 5’-CGCTTCCTGAACTTGTCC-3′ |
R: 5’-GGTTGTCACCTGCTTCCA-3′ | |
TLR4 | F: 5’-CTATCATCAGTGTATCGGTG-3′ |
R: 5’-CAGTCCTCATTCTGGCTC-3′ | |
NF-κB | F: 5’-ATTCAACAGATATACCACTGTCAAC-3′ |
R: 5’-TGTTCTTCTCACTGGAGGCA-3′ | |
U6 | F: 5’-GCTTCGGCAGCATATACTAAAAT-3′ |
R: 5’-CGCTTCACGAATTGTCATTGCGT-3′ | |
β-actin | F: 5’-AGCCATGTACGTAGCCATCC-3′ |
R: 5’-CTCTCAGCTGTGGTGGTGAA-3’ |