Fig. 3

TRPM3 activity after ononetin modulation. Data were obtained under whole-cell patch clamp conditions. a. A representative time-series of current amplitude at + 100 mV and − 100 mV showing the effect of 10 μΜ ononetin on ionic currents in the presence of PregS in isolated NK cells from HC. b I–V before and after application of ononetin in a cell as shown in (a.). c A representative time-series of current amplitude at + 100 mV and − 100 mV showing the effect of 10 μΜ ononetin on ionic currents in the presence of PregS in isolated NK cells CFS/ME patients. d. I–V before and after application of ononetin in a cell as shown in (c.). e. f Scatter plots representing change of each current amplitude before and after ononetin application in all NK cells from HC and CFS/ME patients. Each cell represented as red lines had reduction in currents by ononetin. g Table summarizing data for sensitive and insensitive cells to 10 μΜ ononetin in HC (N = 6; n = 33) compared to CFS/ME patients (N = 6; n = 28). Data are analysed with Fisher’s exact test. Abbreviations: CFS/ME, chronic fatigue syndrome/myalgic encephalomyelitis; HC, healthy controls