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Fig. 2 | Molecular Medicine

Fig. 2

From: RSPO3 impairs barrier function of human vascular endothelial monolayers and synergizes with pro-inflammatory IL-1

Fig. 2

Inter-cellular gaps in vascular endothelial monolayers treated with RSPO3 or IL-1β. a Immunofluorescence staining of VE-cadherin (red) and DAPI staining of nuclei (blue) in HCAEC either untreated, or treated with RSPO3, IL-1β and combined IL-1β and RSPO3 for 6 h. Microphotographs were taken using a Zeiss Axioskope equipped with Axio-CamMRm and AxioVision Rel.4.6 software and are representative of three independent experiments run in triplicates. Original magnification, 630×. b Quantification of inter-endothelial gap index and gap size index in HCAEC treated with RSPO3. Data are mean ± S.E.M of three independent experiments run in triplicates, *P < 0.05, ***P < 0.001 versus untreated (none)

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Molecular Medicine

ISSN: 1528-3658