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Fig. 5 | Molecular Medicine

Fig. 5

From: Asymmetric expression of H19 and ADIPOQ in concave/convex paravertebral muscles is associated with severe adolescent idiopathic scoliosis

Fig. 5

miR-675-5p directly targeted the 3’ UTR of ADIPOQ. a Tissue samples were processed for assessment of expression of miR-675-5p as described in Materials and Methods. miR-675-5p showed a significantly higher expression in convex-sided muscle tissue in AIS patients (n = 60). Results are expressed as mean ± SEM, and the relative expression of miR-675-5p is represented as fold change related to the convex side. b Correlation analysis identified that the expression level of miR-675-5p in muscle tissues was positively correlated with that of H19 in AIS patients (n = 60). c HEK293T cells were co-transfected with miR-675-5p mimics/mimic NC and plasmid with wild type or mutant luc-3’UTR of ADIPOQ. Luciferase values were normalized to those of Renilla luciferase and expressed as fold-induction relative to the basal activity. Co-transfection of luc-3’UTR but not mutant miR-675-5p luc-3’UTR with a miR-675-5p mimic significantly impairs luciferase expression in HEK293T cells. The experiments were performed three times. Results are expressed as mean ± SEM. **, P < 0.01 versus luc-3’UTR. For (d, e), HSkMSC cells were subjected to transfection and differentiation induction as indicated. After 72 h, total cellular proteins were extracted from harvested cells. Levels of ADIPOQ protein were assessed with Western blot using anti-ADIPOQ antibody, as described in Materials and Methods. Left panels illustrate the autoradiographs of representative immunoblots; right panels show densitometric analysis of the immunoblot data (normalized to beta-actin). The experiments were performed three times. Results are expressed as mean ± SEM. *, P < 0.05 versus NC/Mock. f Correlation analysis identified that the expression level of miR-675-5p in muscle tissue samples was negatively correlated with that of ADIPOQ mRNA in AIS patients (n = 60)

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