Fig. 1

LIF induces MOG mRNA with a bell-shaped dose-response curve and inhibits EPO-induced MOG expression. Cells cultured for 1 day in growth medium (GM) were switched to differentiation medium (DM). After 3 h in DM the cells were treated with the indicated concentrations of LIF (a) or with or without EPO (10 ng/ml) and LIF (10 ng/ml, panel b; 0.2 ng/ml, c). MOG gene expression was measured by RT-qPCR at day 3 of differentiation. Results are expressed as fold change (FC) vs one of the control samples (no LIF in panel a and ctr in b and c). Data are the mean ± SD of seven samples from two independent experiments assayed in duplicate (a) or of quadruplicate samples assayed in duplicate and representative or five (b) or two (c) independent experiments; * P < 0.05, **P < 0.01, ***P < 0.001 vs control; § P < 0.01 vs EPO alone by two-tailed Student’s t-test