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Fig. 3 | Molecular Medicine

Fig. 3

From: Leukemia inhibitory factor inhibits erythropoietin-induced myelin gene expression in oligodendrocytes

Fig. 3

Genes regulated by LIF at 20 h. Cells cultured for 1 day in GM were switched to DM; after 3 h EPO with or without LIF was added for 20 h. a Flow chart. Genes regulated by differentiation, EPO and LIF were selected as in the legend to Fig. 2. Positive regulators induced by EPO and inhibited by LIF are highlighted in green (16, of which 7 induced also by differentiation). Negative regulators induced by LIF and unchanged by differentiation or EPO (256) or decreased by differentiation (69) or by EPO (2) are highlighted in red. b Venn diagrams showing positive regulators inhibited by LIF (left; EPO-induced genes unchanged or induced by differentiation, 9 and 7 respectively, green arrows) and negative regulators induced by LIF (right; 256 unchanged by differentiation or EPO; 69 and 2 decreased by differentiation or EPO respectively, red arrows). The genes changed in opposite directions by EPO and differentiation are not included in b. These are: 8 genes increased by differentiation but decreased by EPO and 21 genes increased by EPO but decreased by differentiation (a). In addition, the left diagram (positive regulators) does not include the genes decreased by LIF but also decreased by differentiation or EPO (4 + 1 + 48 + 131 + 27 = 211; a). The right diagram (negative regulators) does not include the genes increased by LIF but also increased by EPO or differentiation (13 + 1 + 75 + 5 + 43 = 137; a). Dif, differentiated; undif, undifferentiated. c Gene-gene interaction network of the putative negative regulators increased by LIF at 20 h. All the genes increased by LIF and decreased by differentiation alone (69 genes, a) or by EPO alone (2 genes, a) were analysed with the STRING software as described in the legend to Fig. 2. The full list of all the 71 genes is reported in Additional file 4

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